Journal: Oncotarget

Article Title: Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis

doi:

Figure Lengend Snippet: Up-regulated and down-regulated genes in the liver of EtOH-treated Tg rats

Article Snippet: Thereafter a polyclonal rabbit antibody against GST-P was used with biotin-conjugated anti-rabbit IgG and Cy5-labeled streptavidin (Thermo Fischer Scientific Inc.), and finally the section was incubated with an Alexa Fluor 350 conjugated rabbit anti-DUSP1 polyclonal antibody (Bioss Inc., Boston, MA).

Techniques:

Journal: Oncotarget

Article Title: Connexin 32 dysfunction promotes ethanol-related hepatocarcinogenesis via activation of Dusp1-Erk axis

doi:

Figure Lengend Snippet: Levels of Dusp1 mRNA A. and protein B. in whole liver tissues measured by quantitative RT-PCR and western blotting, respectively. Data of quantitative RT-PCR are presented as mean ± SD, n = 4 per groups. **, ***: P < 0.01 and 0.001 respectively. Representative image of laser microdissected tissue (upper) and Dusp1 mRNA level in GST-P positive foci as determined by quantitative RT-PCR (lower) C. Immunohistochemical staining for Dusp1 in GST-P positive foci of Tg and Wt rats that received 5%EtOH (upper) and the intensity score of Dusp1 in GST-P positive foci as compared to Wt-control rats (lower) D. Data are presented as mean ± SD, n = 5 per group, 10 foci/rat. *: P < 0.05.

Article Snippet: Thereafter a polyclonal rabbit antibody against GST-P was used with biotin-conjugated anti-rabbit IgG and Cy5-labeled streptavidin (Thermo Fischer Scientific Inc.), and finally the section was incubated with an Alexa Fluor 350 conjugated rabbit anti-DUSP1 polyclonal antibody (Bioss Inc., Boston, MA).

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Staining

Journal: International Journal of Molecular Sciences

Article Title: NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway

doi: 10.3390/ijms19061625

Figure Lengend Snippet: Involvement of MKP-1/ERK1/2 pathway in NSC 95397-induced p21 expression and caspase-3 activation in human colon cancer. Representative Western blots showing ( A ) the expression of Cdc25A, the phosphorylation of Cdk1 at Tyr15; ( B ) the expression of MKP-1 and ERK1/2, and the phosphorylation of ERK1/2 at Thr202/Tyr 204 in SW480, SW620, and DLD-1 cells treated with 10 μM NSC 95397 for 6 h, with actin as loading control; ( C ) representative Western blots showing expression of p21 and cleaved caspase-3 in colon cancer cells that were pre-incubated with 20 μM U0126 for 2 h and then treated with 10 m μM NSC 95397 for 6 h.

Article Snippet: The following antibodies were used: rabbit monoclonal anti-p21 (Cell Signaling Technology #2947, Danvers, MA, USA), rabbit monoclonal anti-CDK4 (Cell Signaling Technology #12790), mouse monoclonal anti-CDK6 (Cell Signaling Technology #3136), rabbit polyclonal anti-Phospho-Rb (Ser795) (Cell Signaling Technology #9301), rabbit monoclonal anti-Phospho-Rb (Ser807/811) (Cell Signaling Technology #8516), mouse monoclonal anti-Caspase-9 (Cell Signaling Technology #9608), rabbit monoclonal anti-Cleaved Caspase-9 (Cell Signaling Technology #7237), rabbit monoclonal anti-Caspase-3 (Cell Signaling Technology #9665), rabbit monoclonal anti-Cleaved Caspase-3 (Cell Signaling Technology #9664), rabbit monoclonal anti-Caspase-7 (Cell Signaling Technology #12827), rabbit monoclonal anti-Cleaved Caspase-7 (Cell Signaling Technology #8438), rabbit polyclonal anti-PARP (Cell Signaling Technology #9542), rabbit monoclonal anti-Cleaved PARP (Cell Signaling Technology #5625), rabbit polyclonal anti-cdc25A (Cell Signaling Technology #3652), rabbit polyclonal anti-Phospho-cdc2 (Tyr15) (Cell Signaling Technology #4539), rabbit polyclonal anti-MKP-1 (Bioworld Technology #BS1677, St. Louis Park, MN, USA), rabbit polyclonal anti-ERK1/2 (GeneTex #GTX59618, Irvine, CA, USA), rabbit polyclonal anti-Phospho-ERK1/2 (GeneTex #GTX61126), and mouse monoclonal anti-Actin (Merck Millipore #MAB1501, Burlington, MA, USA).

Techniques: Expressing, Activation Assay, Western Blot, Incubation

Journal: International Journal of Molecular Sciences

Article Title: NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway

doi: 10.3390/ijms19061625

Figure Lengend Snippet: A schematic showing that the cytotoxic effect of NSC 95397 on colon cancer cells is activated by inhibiting MKP-1 activity followed by ERK1/2 activation. Lines ending in arrows indicate promotion and T-bars represent inhibition.

Article Snippet: The following antibodies were used: rabbit monoclonal anti-p21 (Cell Signaling Technology #2947, Danvers, MA, USA), rabbit monoclonal anti-CDK4 (Cell Signaling Technology #12790), mouse monoclonal anti-CDK6 (Cell Signaling Technology #3136), rabbit polyclonal anti-Phospho-Rb (Ser795) (Cell Signaling Technology #9301), rabbit monoclonal anti-Phospho-Rb (Ser807/811) (Cell Signaling Technology #8516), mouse monoclonal anti-Caspase-9 (Cell Signaling Technology #9608), rabbit monoclonal anti-Cleaved Caspase-9 (Cell Signaling Technology #7237), rabbit monoclonal anti-Caspase-3 (Cell Signaling Technology #9665), rabbit monoclonal anti-Cleaved Caspase-3 (Cell Signaling Technology #9664), rabbit monoclonal anti-Caspase-7 (Cell Signaling Technology #12827), rabbit monoclonal anti-Cleaved Caspase-7 (Cell Signaling Technology #8438), rabbit polyclonal anti-PARP (Cell Signaling Technology #9542), rabbit monoclonal anti-Cleaved PARP (Cell Signaling Technology #5625), rabbit polyclonal anti-cdc25A (Cell Signaling Technology #3652), rabbit polyclonal anti-Phospho-cdc2 (Tyr15) (Cell Signaling Technology #4539), rabbit polyclonal anti-MKP-1 (Bioworld Technology #BS1677, St. Louis Park, MN, USA), rabbit polyclonal anti-ERK1/2 (GeneTex #GTX59618, Irvine, CA, USA), rabbit polyclonal anti-Phospho-ERK1/2 (GeneTex #GTX61126), and mouse monoclonal anti-Actin (Merck Millipore #MAB1501, Burlington, MA, USA).

Techniques: Activity Assay, Activation Assay, Inhibition

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: The expression of CCDC80 protein in normal ovary (a) and (b) and ovarian carcinoma (c) and (d) tissues through immunohistochemical (IHC) staining.

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: Scatter plots of CCDC80 protein (a) and mRNA (b)–(i) expression of OVCA and the corresponding normal controls.

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: The receiver operating characteristic (ROC, (a)–(i)) and sROC (j) curves of CCDC80 in OVCA and Deek's test for publication bias test (k).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques:

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: Combined standard mean difference (SMD, (a)) of CCDC80 expression between OVCA and non-OVCA group and Egger's test (b) for publication bias test (in mRNA group, TCGA_GTEx_ovary was a cohort of RNA-seq, and other cohorts began with “GSE” were gene chip cohorts).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Expressing, RNA Sequencing

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: The relationship between CCDC80 expression and the fraction of immune cell infiltration in OVCA based on CIBERSORT algorithms.

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: The boxplots visualizing the single-sample gene set enrichment analysis (ssGSEA) in high- and low- CCDC80 expression group in OVCA based on immunocyte-related gene sets (a) and immune function-related gene sets (b) ( ∗∗∗ , P < 0.0001; ∗∗ , P < 0.001; ∗ , P < 0.05; ns, P ≥ 0.05).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Expressing

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: KEGG enrichment plots of the intersection genes from CCDC80 positively related coexpressed genes (CEGs) and downregulated differentially expressed genes (DEGs) (a), and CCDC80 negatively related CEGs and upregulated DEGs (b).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques:

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: A volcano plot shows the results of GSEA based on the OVCA cell lines and enrichment plots of immune-related gene sets in low- CCDC80 group (b) and (c) and high- CCDC80 group (d) and (e).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques:

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: Seqlogos of the motifs of transcription factor NR3C1 (a) and PBX3 (b); ChIP-seq peak (see red arrow) of NR3C1 in the upstream of the transcription start site of CCDC80 (c); and the yellow arrow indicates the transcription direction of CCDC80 ; no ChIP-seq peak of PBX3 in the upstream of the transcription start site of CCDC80 (d).

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: ChIP-sequencing

Journal: International Journal of Genomics

Article Title: Downregulation of the Coiled-Coil Domain Containing 80 and Its Perspective Mechanisms in Ovarian Carcinoma: A Comprehensive Study

doi: 10.1155/2021/3752871

Figure Lengend Snippet: Violin plots visualizing the differences of estimated half maximal inhibitory concentration (IC50) of compounds between high- and low- CCDC80 expression group.

Article Snippet: Afterward, immunohistochemical (IHC) staining conducted using CCDC80 polyclonal antibody (biorbyt, orb216089, rabbit-anti-human) with 150 OVCA tissues and 46 non-OVCA tissues from clinical samples and tissue microarrays.

Techniques: Concentration Assay, Expressing